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1.The ion exchange method was used to purify and enrich glutathione from wheat germ extract permeating liquor through 5nm ceramic membrane . The result of comparative experiments showed that Amberlite 252 macroporous ion exchange resin was the most appropriate type, and that 1 mol/L HCl liquor was the most appropriate eluant. The volume of permeating liquor decreased 55 % after ion exchange processing and the concentration of glutathione nearly increased two times.
实验选用了离子交换分离法,对经膜分离后得到的小麦胚水溶性提取物的透过液中的谷胱甘肽进行了纯化和富集,通过对比试验的分析结果表明:最佳离子交换树脂是Amberlite 252大孔型树脂,最佳洗脱剂是1mol/L HCl溶液,透过液经过离子交换过程之后体积减少55%,谷胱甘肽浓度提高近2倍。收藏指正
2.Owingto usong microresins with diameters of 30-60 micron,the technology ofpressurized ion exchange and high temperature(75℃),as against thetraditionalion exchange technology,the method improves the followingtechnogical condions:the concentration of displacer(EDTA)enhanees from0.015M to 0.100M,linear flow rate of displacer from 0.5-1.0cm/min. to10cm/min.
如排代剂浓度、pH和 HEPT,由于使用直径为30~60μm 米的微粒树脂,加压离子交换技术和高温,同常温常压离子交换法比较:排代剂 EDTA 浓度由0.015M 提高到0.100M,排代剂线性流速由0.5~1.0cm/min 提高到10cm/min;收藏指正
3.amino acids were determined by ion exchange HPLC with OPA post column derivatization. When the concentration of amino acid were 50~180μmol/L (the tryptophan was 61.5~221.4μmol/L), the correlation coefficients between the peak area of amino acid and the concentration of amino acid were above 0.96.The recoveries of 20 amino acid added were 95.7%~104.2%.
应用邻苯二甲醛(OPA)柱后衍生、离子交换高效液相色谱法同时测定20种氨基酸,氨基酸浓度在50~180μmol/L(色氨酸浓度为61.5~221.4μmol/L)时,其峰面积和氨基酸浓度的线性相关系数均大于0.96,20种氨基酸的加标回收率在95.7%~104.2%。收藏指正
4.The concentration and separation of Np and Pu in TRPO process are studied by anion exchange method.
用阴离子交换法研究了TRPO流程中镎和钚的浓缩和分离。收藏指正
5.Effect of Different FSB Concentration and First-time Exchange of Total Volume Medium on Proliferation of Bone Marrow Stromal Cells from GFP Transgenic Mouse in vitro
血清量及首次换液时间对荧光小鼠骨髓基质细胞体外增殖的影响收藏指正
6.The acidic property of sodium montmorillonite changes after its interlayer ions being replaced by various metallic ion exchange and heat treated under different conditions. Its surface acidic property can be evalualed by CVL coloration method. As shown by the results, type and concentration of metallic ions and temperature of heat treatment exercise influence in different degrees.
Na蒙托石的层间离子被各种金属离子交换取代后,经不同条件的热处理,表面酸性发生了变化.用CVL显色法评价其表面酸性,结果表明金属离子类型、浓度以及热处理温度等对表面酸性有不同程度的影响.以交换后的Ni蒙托石为一元酸酯化催化剂,具有较高的催化活性.收藏指正
7.2. Purification of HSP70 — peptide complex: the mixed protein was separated by affinity chromatography on concanavalin A — Sepharose and DEAE—Sephacel exchange chromatography. 3. The qualitative and quantitative analysis of HSP70 — peptide complex: the molecular weight and identity of the HSP70 was confirmed by SDS—PAGE and Western blot, the concentration was measured by the means of Bradford.
本试验的研究目的就是应用快速蛋白液相色谱(FPLC)系统从肝癌组织中建立分离和纯化HSP70—肿瘤肽复合物,并研究其对肝癌细胞系HepG—2增殖的影响和其对HepG—2中JNK和c—Jun表达的影响。收藏指正
8.In the Jianan poetry the image of "the sad wind" is a special image with the bright time characteristic. This kind of image is mainly deep by the emotion, concentration on the exchange of art situation.
建安诗歌中的“悲风”意象是一个具有鲜明时代特色的特殊意象,这种意象主要以情感的真挚深厚、普遍集中以及艺术上情景交融的高质量见长。收藏指正
9.It is generally believed that CGs produce positive inotropic actions in failure heart via the inhibition of Na+,K+-ATPase activities which results in the elevation of bulk intracellular Na+ concentration([Na+]), and subsequent increase of intracellular Ca2+ concentration ([Ca2+]i) via Na+-Ca2+ exchange, thus increasing the uptake and subsequent release of Ca2+ by the sarcoplasmic reticulum (SR).
一般认为:强心苷抑制衰竭心肌细胞膜上的Na+,K+-ATP酶活性,使细胞内钠离子浓度([Na+]i)增加,通过Na+/Ca2+交换,引起Ca2+的净内流,并促进肌浆网对钙的摄取及随后的钙释放,心肌收缩力随之增加。收藏指正
10.Very low concentration of Co, Cu, Cd, Ba, Mn, Ni, Pb in water samples at Larsemann Hills and East Antarctic was determined by off line preconentration with chelating cation exchange resin D401 coupled with ICP MS.
采用脱线螯合阳离子树脂 (D40 1 )将待分析元素预富集并与基体元素分离 ,采用电感耦合等离子体质谱法 (ICP MS)测定了南极拉斯曼丘陵地区环境水样中的难于或不能直接检测的超痕量元素Mn ,Co ,Ni,Cu ,Cd ,Ba和Pb ;收藏指正
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