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1.Epidermal explant culture was applied, and effects of AK auto Ab with variable dose on rate of epidermaration were observed. Brd U incorporation assay was used to investigate the extent of mitosis.
方法 采用贴壁法体外培养皮肤 ,比较观察不同浓度 AK auto Ab对表皮形成速率的影响 ,并采用 Brd- U掺入法测定各组细胞增殖率 .收藏指正
2.The effects of different basic media(1/3 MS,1/2 MS,MS,VW), different organic compounds (coconut milk, potato, banana and apple ) and hormone on the propagation of Phalaenosis PLB (Protocorm like-body) were studied by adopting phalaenopsis PLB for explant culture.
以白色花系蝴蝶兰(Phalaenopsis)的原球茎为外植体进行培养,比较不同基本培养基(1/3MS,1/2MS,MS,改良VW),有机添加物(椰乳,香蕉,马铃薯,苹果)和植物激素(BA,NAA)等因子对影响蝴蝶兰原球茎(Protocormlikebody,简称PLB)增殖效果进行了研究。收藏指正
3.Result shew: the number of chondrocyte of enzyme digestion was more than that of explant culture,The viability of chondrocytes isolated with collagenase typeⅡconfected by serumfree medium were markedly higher than that of PBS (free of Ca~ 2+ ,Mg~ 2+ ). There was statistical discrepancy between the two enzyme digestion groups(P<0.05).
结果表明:在获取细胞的数量上,酶消化法明显优于组织块法; 在成活率比较试验中,Ham-F12配制组明显高于常规的PBS液(无Ca2+、Mg2+)配制组,两组之间存在显著差异(P<0.05);收藏指正
4.[Methods] 20 placental samples of pregnant women with ICP and 20 normal term placental samples were investigated the expression of HIF-1α and -2α mRNA by RT-PCR. Protein from villous explant culture under hypoxia condition (n=8each) was probed for HIF-1α, HIF-2α and P53 by immuno-histochemistry methods.
[方法] 采用RT-PCR方法检测ICP患者和正常晚孕妇女胎盘上HIF-1αmRNA,HIF-2αmENA的表达;收藏指正
5.The explant tissue culture was compared with the typsin digestion culture.
对比了组织块培养和消化培养两种培养方法。收藏指正
6.Studies on Browning Factors of Explant and Proliferation Culture of Walnut(Juglans Regia L.)
核桃外植体褐化影响因素及增殖培养研究收藏指正
7.Advances of explant browning in plant tissue culture
植物组织培养外植体褐变的研究进展收藏指正
8.Studies on the Methods to Prevent Explant from Browning in Tissue Culture of Diospyros kaki
柿树组织培养防止外植体褐变的研究收藏指正
9.Research Progress on Mechanism of Explant Changing to Brown in Plant Tissue Culture
植物组织培养过程中外植体褐变机理研究进展收藏指正
10.Such a piece of transplanted tissue is called an explant, and its placement on the culture medium is called inoculation.
这种被移植的组织称外植体,将它放置在培养基上称为接种。收藏指正
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