explant culture
3.Result shew: the number of chondrocyte of enzyme digestion was more than that of explant culture,The viability of chondrocytes isolated with collagenase typeⅡconfected by serumfree medium were markedly higher than that of PBS (free of Ca~ 2+ ,Mg~ 2+ ). There was statistical discrepancy between the two enzyme digestion groups(P<0.05).
4.[Methods] 20 placental samples of pregnant women with ICP and 20 normal term placental samples were investigated the expression of HIF-1α and -2α mRNA by RT-PCR. Protein from villous explant culture under hypoxia condition (n=8each) was probed for HIF-1α, HIF-2α and P53 by immuno-histochemistry methods.

