extend chromatography
1.Conditions of chromatography were:Agilent Extend-C18(4. 6×250mm) was used as column, and mobile phase was composed of methanol: water: glacia acetic acid(45 : 55 :0. 1),detection wavelength was at 280nm, the flow rate was 1. 0ml/min, column temperature was at 30 ℃ , the injection volume was 10цl.
