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1.The pathogenic fungi Gaeumannomyces graminis var.tritici(Ggt) was cultured in modified MS medium(26 ℃,150 r/min) with different combinations of carbon and nitrogen sources. The enzyme activity in the culture filtrate was detected by 3,5-dinitrosalicylic acid(DNS) in 7,14,21 d after culturing the fungus.
利用改进的MS液体培养基,通过改变碳、源组合,分别在振荡培养小麦全蚀病菌7,14,21 d时(26℃,150 r/min),用3,5-二硝基水杨酸(DNS)比色法测定培养滤液中的β-1,3-葡聚糖酶活性。收藏指正
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