filtration medium
1.A needled dacron woolen cloth with good filtration ability is used as filtration medium,which provides high filtration efficiency, and good dust—cleaning effect. The dust filtration efficiency is up to 99.5— 99.8%. The pressure loss of the unit is 1.2—1.4 kPa at the wind velocity of 2—3m/min.
2.coli BL21(DE3) transformed with pET 29a(+)/ATF was induced with IPTG to overexpress recombinant human ATF (rhATF) as insoluble inclusion body. The amount of rhATF expressed accounts for 20% of total bacterial protein. After puritication of inclusion body, renaturation, CM cation exchange chromatography and Superdex G 75 gel filtration, 15 mg rhATF was obtained from one liter of culture medium with homogeneity greater than 95%.
3.The vacuum filtering is isobarically, so according to the Ruth Filtration Equation, the degree of the system vacuum, filtering time, filtering area, filtering medium, the feature of the slurry and the uniform rate of the slurry are main factors affecting the working efficiency of the Vacuum Vertical Plate Filters.
