flask shaker
4.They were inoculated into a 250ml Erlenmeyer flask containing 50 ml of the media with Ca 3(PO 4) 2 or organophorus pesticide as the only phosphorus source. The flasks were cultivated on a rotary shaker at 30℃ for 4 days under agitation of 180 r/min, then phosphorus in the medium were measured by different methods.
6.The optimum conditions were as follows:the 300mL Erlenmeyer flask containing 150mL of liquid medium was inoculated with 7.5mL of mycelial fragments and the medium was supplied with lignin at a concentration of 0.1%(initial pH8.5). The cultures was incubated at 28℃ on rotary shaker(150r/min) for 4～5 days. The maximum enzyme activity was 20184IU/L.
