fluorescent assay
2.After treatment with AZT, the telomerase activity in SMMC-7721 cells was detected by real-time fluorescent quantitative TRAP (FQ-TRAP) assay;
3.Detecting serum samples AMA-M2, ANA, ENA, ds-DNA, 17 cases of PBC patients and 32 cases of non-PBC patients and 32 cases health control by EUROASSAY, Indirect Immunoassay Fluorescent, Dot Immunogold Filtration Assay and doing statistics and analyzing on the detecting result.
4.The tail extent, tail inertia, percentage of tail DNA and Olive tail moment are selected as parameters, which could be measured by the fluorescent pictures of single cell gel electrophoresis (SCGE) and IMI 1.0 comet assay analysis software.
5.Estrogen receptor (ER) in breast cancer tissue from 52 patients and progesterone receptor (PR) from 23 patients were determined by a fluorescent histochemical method (FHC) with 17 -estradiol-6-CMO-BSA-FITC conjugate and Destran-Coated charcoal adsorption (DCC) assay. The result showed that the FHC assay had correlation with DCC assay in determiniing ER and PR The concordance about ER and PR between the two methods were 70% and 78%, respectively.
6.Enzyme-linked immunosorbent assay (ELISA) and fluorescent bead immunoassay (FBI) were used to detect the concentration of rhLTα-Da in blood and urine.
7.Methods:Drug sensitivity and proliferation of leukemic cells in vitro were determined by leukemic cell colony forming unit (CUF L),MTT drug sensitive test, percentage of S phase cells in cell cycle (S%), fluorescent index(FI) and drug resistant index (DRI) by detecting intracellular daunorubicin, expression of P 170 glycoprotein by APAAP assay, and abundance of bcl XL mRNA by semiquantitative reverse transcription polymerase chain reaction (RT PCR).
8.METHODSMGC803 cell growth inhibitio n was measured by MTT assay. Flow cytometry and acridine orange fluorescent stai ning method were used to determine the induction of apoptosis and the change of cell cycle.
9.Methods T lymphocytes from C57/BL spleens were isolated by nylon absorbance assay and cells were labeled by CFSE, a cell membrane-inserted fluorescent dye. Aliquots (2×107) of T lymphocytes were administrated intravenously intoγ-irradiated (5Gy) female BALB/c mice.
10.? In this study,DNA hybridization,cell adherence assay and fluorescent actin staining test were used to study the pathogenic factors of EPEC isolated from children with diarrhea by detection of eaeA,bfpA,s1t1 and s1t2 genes,adherence pattems to cultured HEp?2 cells and attaching and effacing(AE)lesions.
