focusing electrophoresis
1.After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.
2.Objective? This paper reports a potent platelet aggregation inhibitor which was an acidic PLA 2 purified from ?Ophiophagus hannah ?venom ?Methods? Acidic PLA 2 was obtained from Guangdong ?Ophiophagus hannah? venom by column chromatography and rechromatography on CM Sephadex C 25, Sephadex G 75, DEAE Sephadex A 25 The purity was tested by polyacrylamid gel electrophoresis ,gel isoelectric focusing electrophoresis and SDS electrophoresis;
3.The molecular weight of the antigen obtained was 15100 dalton as measured with SDS--PAGE By means of isoelectric focusing electrophoresis, it was found to consist of two subunits and their isoelectric points are 5.32 and 5.54 respectively.
4.The 16 given wild rice species belong to the genomes AA, BB, CC, CCDD, BBCC, EE, or FF. The results of esterase isozymes isoelectric focusing electrophoresis indicate that there was individually a special pattern of esterase among Ser. sativae, Ser.
5.ResultsThe purified protein BDA showed a single band by SDS-PAGE with a molecular weight of 15 200,and BDB showed the same character but the molecular weight was 14 600.The isoelectric point of BDA was pH 3.97 and BDB was pH 4.61 by the isoelectric focusing electrophoresis.
6.It was separated into 6-7 bands by thin layer isoelectric focusing electrophoresis, and their isoelectric points were 3.23-5.23. Immunogenic test showed that the CA (10-20 μg/ml) gave rise to positive reactions with specific hyperimmune sera against 5 strains of CJ by ELISA.
7.When the isoelectric point of the polyhedrin was determined by isoelectric focusing electrophoresis, 7bands appeared, with a major band at p15.78±0.02 and minor bands at p15.35—6.25. The molecular weight of the polyhedrin subunit is 28000±500 dalton by SDS-PAGE.
8.Proteins and 5 isoenzymes(LDH,MDH,SOD and ACP )of the muscle─stage larvae of a strain from Tianjin pig(TJP),a strain of unknown origin(X) and a strain Changchun dog(CCD)of Trichinella spiralis were analyzed using polyacrylamide gel─isoelectric focusing electrophoresis (PAGE─IEF).
9.Four insect toxic components were obtained from the crude venom of scorpion Buthus martensii Karch by Sephadex G-50 gel filtration and rechromatogra-phy on sp-Sephadex C-25. They induced excitatory paralysis symptoms to cockroach Periplaneta americana. The results of isoelectric focusing electrophoresis and SDS PAGE of two insect toxic fractions showed they are homogeneous. Named BmK IT-Ⅰ and BmK IT-Ⅱ .
10.The isoelectric point of two kinds of DPO were both 5.49 by means of isolectric focusing gel electrophoresis(IEF);
