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1.Alkaline PhoPhatases (ALP) were extracted and purified from chicken skeleton, liver and intestine with butanol extraction and chromatography on DEAE-cellulose (DE-52) and concanavalin ASepharose 4B. Skeleton and liver tissues ALPs were separated with polyacrylamide gradient gel electrophoresis into two components, with molecular weights of 154000 and 353000(skeleton), 187000 and 353000(liver).
用正丁醇抽提、DEAE纤维素(DE—52)和Con·A—Sepharase 4B柱层析等方法从鸡的骨、肝和肠组织中提取碱性磷酸酶(ALP)。 提纯倍数分别为41倍(骨)、391倍(肝)和130倍(肠)。收藏指正
2.Methods The changes of the erythrocyte filtration index( EFI) ,erythrocyte membrane skeleton structure, spectrin dimers(SP D) and spectrin tetramers(SP T) were measured in 64 patients and 39 controls by using the DXC 300A erythrocyte deformability apparatus,JEM 1200EX transmission electron microscope and SDS gel eletrophoretic analysis.
②方法采用DXC-300A型核孔膜红细胞变形能力测定仪、JEM-1200EX透射电镜和SDS-聚丙烯酰胺凝胶电泳法,检测了64例NIDDM病人和39例正常人红细胞滤过指数(EFI)、红细胞膜骨架结构和收缩蛋白二聚体(SP-D)和四聚体(SP-T)的变化。收藏指正
3.The main cause of corrosion on glass fibre in portland cement paste is the reactionbetween Ca(OH)_2, precipitated from the hydration of cement, and Si-O skeleton of glassfibre and then produces hydrated calcium silicate gel of C-S-H(I)type, while imperfectionson surface layer of glass fibre accelerate this process.
玻璃纤维在普通硅酸盐水泥石中受侵蚀的主要原因是水泥水化时析出的Ca(OH)_2与玻璃纤维的硅氧骨架反应形成了CSH(Ⅰ)类型的水化硅酸钙,而玻璃纤维的缺陷则加速了这一过程。 在C-A-CS-H~(**)系中C_3A.收藏指正
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