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1.Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis.
目的:基于生物信息学分析构建人ID4基因启动子表达载体,以其作为研究ID4基因启动子表达调控分析的工作基础。收藏指正
2.A Fab antibody gene was constructed on the basis of the reconstruction of the linker of a human anti TNF α ScFv gene.
在获得人源性抗人TNF α单链抗体 (ScFv)基因序列的基础上 ,对ScFv的连接肽部分进行基因改造 ,并构建了Fab抗体基因。收藏指正
3.Conclusions GAD67 gene had been cloned from rat brian using RT-PCR and T vector techniques. The success of cloning of Glutamic acid decarboxylase 67 gene lays a good basis for construction of a recombinant expressed.
结论　采用RT -PCR和T载体技术获得了大鼠脑组织中的谷氨酸脱羧酶GAD6 7基因克隆 ,为该基因的体外表达打下基础 .收藏指正
4.Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2′s expression.
克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。收藏指正
5.Conclusion The bladder TCC cell line with stable high expression of UPII gene has been successfully established through gene transfection method,which was a basis for further exploring the roles of UPII gene in TCC biological behaviors and potential strategies for targeted biological therapy of TCC.
结论　通过基因转染方法建立了稳定高表达UPII基因的膀胱TCC细胞株 ,为进一步探讨UPII基因在膀胱TCC生物学行为中的作用及其靶向生物治疗策略奠定了基础。收藏指正
6.The cDNA fragments of the E2 gene were sequenced,and on the basis of the amino acid sequences of China strain, Brescia strain and Alfort strain,the amino acid sequence encoded by the E2 gene was deduced.
经转化、筛选、鉴定后 ,测定了核苷酸序列 ,根据C株、Brescia株和Alfort株确定起始氨基酸三联体的正确位置后进行 gp5 5氨基酸序列推导 ,并进行同源性比较。收藏指正
7.The double muscle beef, Belgian Blue was also caused by the natural deletion ofGDF-8.In order to study further natural functions of the porcine myostatin gene of which were obtain pGEM-3Z cloning vector in myostatin gene and to establish theory basis of molecular breeding of porcine, two pairs of upper, lower specific PCR primers which containing EcoR I and BamH I restriction enzyme sites was designed.
为进一步研究myostatin基因的结构与功能的关系、进行该蛋白的基因工程研究以及探索免疫蛋白的研制，本研究以克隆在pGEM-3Z上的猪myostatin基因cDNA为研究对象，利用Oligo5.0软件设计出两对上、下游特异性引物，并分别引入EcoRI和BamHI两酶切位点。收藏指正
8. Conclusion The construction of AMA 1(Ⅲ) gene expression clone, molecular biology, is the basis of AMA 1(Ⅲ) expression in pichia pastoris.
④结论　用分子生物学方法重组构建的AMA 1(Ⅲ )毕氏酵母真核表达克隆 ,为高效表达AMA 1(Ⅲ )及其活性鉴定奠定了基础收藏指正
9.3.Transforming IPX gene to GSDUR and transgenic plants was obtainedOn the basis of the above mentioned genetic transformation system, chimeric gene PSAG12-IPT was transformed to the embryonic callus tissue of GSDUR through Agrobacterium-mediated method.
3、IPT基因遗传转化谷秆两用稻，获得转基因植株收藏指正
10.Firstly it constructed a curved face equation, and then did self-learning by adjusting power gene and center and width of the radial basis function with restriction condition on reflect function, finally gained satisfactory curved face equation.
该算法先采用网络构造一个曲面方程，再利用反射函数作为约束条件，通过调整权因子和径向基函数中心和宽度对网络进行自学习，得到一个满意的曲面方程。收藏指正
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