2.Genelibrary of strain A12was constructed and an alpha amylase gene amyA1was cloned though screening library of strain A12.Gene amyA1was sequenced and expressed in Escherichia coli JM109(DE3).
7.To identify native epitope of N protein, we constructed the ORF7 gene-targeted peptide library and screened by the MAb N3H2. After 3 rounds of biopanning, 3 clones were obtained.