2.Then , partial eDNA of Luffin ~2 was cloned by RT-PCR with two primers ( one was degenerate primer for 5?terminal primerand the other was a generalprimer containing oligo-dT).
3.A pair of generalprimer (ITS5-ITS4) was used to test in amplification of internal transcribed spacer (ITS) region of chromosomal DNA of fruiting bodies of Boletus edulis in Yunnan.