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1.Results Both MCC and IMCC remained serum dependence and stop proliferating in DMEM without serum. Proliferation rate of IMCC and MCC in 5 % serum medium was obvious less than that in 10% and 20% serum serum medium.
结果IMCC和MCC均不能在无血清培养基中生长,但可在含5%血清的培养基中生长,其增殖速度明显低于在10%和20%两种血清浓度中细胞的生长速度,而10%和20%两种血清浓度中细胞的生长速度则较接近。收藏指正
2.Methods: KCs from rat were cultured with non trophoblast method in serum medium containing some regulation factors.
方法 :应用含有新生牛血清并附加多种调节因子的 DMEM- F1 2培养基 ,对大鼠表皮 KCs进行无滋养层法培养。收藏指正
3.We compared the kit with Hayflick and no serum culture-medium.
选用多种有效抑菌剂以省去固体培养基鉴定的步骤,并与Hayflick培养基及无血培 养基进行对比研究。收藏指正
4.Conclusion:The serum-containing medium for culture of CHO cells can be completely substitutedby serum-free media.
结论:无血清培养基可以完全取代含血清培养基用于培养CHO细胞。收藏指正
5.Objective To demonstrate the characterization of keratinocytes cultured in feeder cell layer, serum-containing medium(FSCM) and in a serum-free medium(SFM).
目的对比有饲养层的有血清培养体系(feederlayer,serum—containingmedium ,FSCM)和无血清培养体系(serumfreemedium ,SFM)培养人角朊细胞(keratinocyte ,KC)的特征。收藏指正
6.Methods: Cell culture in serum-free medium, indirect immunofluorescence cytochemistry were used.
方法:采用无血清细胞培养技术,间接免疫细胞化学染色法。收藏指正
7.CONCLUSION:The keratinocytes growed best in serum-free medium supplemented with EGF and BPE.
结论 :细胞在加入表皮生长因子和促进增殖的垂体提取物的无血清培养基中增殖良好。收藏指正
8.Effect of EGF and BPE on the human pituitary adenoma cells cultured in serum-free medium
表皮生长因子和牛垂体提取物对无血清培养的人垂体腺瘤细胞的作用收藏指正
9.Results showed that the keratinocytes growed best in serum-free medium supplemented with EOF and BPE.
结果显示细胞在无血清培养基中生长良好,而加入表皮生长因子和促进增殖的垂体提取物可以进一步改善第四军医大学硕士学位论文细胞的生长状态。收藏指正
10.Methods Rabbit conjunctival epithelial tissues were explanted in serum-free system The morphology, gowth, proliferation and differentiation of outgrow cells were compared with those in serum-containing medium.
方法分别用含胎牛血清培养液和不含血清培养液进行组织块培养,观察两种培养方法在细胞形态、细胞生长情况和增殖及细胞分化方面的差异。收藏指正
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