h37rv
2.Molecular Cloning and Expression of the Immunodominant Protein Ag85A from Mycobacterium tuberculosis H37Rv Strain
3.Results We constructed the plasmid which could highly express Mycobacterium tuberculosis H37Rv ICL.
4.The RNA polymerase activity of H37Rv strain was strongly inhibited by RFD, but not that of the resistant strain.
5.Methods Ag85A gene was amplified by polymerase chain reaction (PCR) from the genome of Mycobacterium tuberculosis H37Rv strain.
6.Methods Amplify Rpf E gene from the genomic DNA of M.tuberculosis H37Rv by PCR,insert into expression vector pET32a(+) and transform to E.
7.Methods Detecting the TB-DNA in 30 strains of Mycobacterium tuberculosis and 4 strains of non- Mycobacterium tuberculosis by PCR-southem blot, and comparing with that of the reference strain (H37Rv).
8.The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction (PCR) from the genomic DNA ofM. tuberculosis H37RV strain;
9.30 isolates and H37Rv had no mutation by SSCP. 19 out of 20 isolates which were resistant to INH were found to have katG mutation by SSCP.
10.coli. Methods The gene encoding protein MPT53 was amplified from mycobacterium tuberculosis H37RV chromosomal DNA by using PCR, then cloned into pMD-T vector after identifying, then cloned pET32a expressing vector, transformed into E. coli BL21. Bcaterial lyastes prepared from IPTG induced cultures were loading SDS-PAGE.
