hemagglutinin(HA)
1.A pseudorabies virus (PRV) transfer vector(pLTK-HA)containing HA gene of H3N2 SIV used tocotransfect with genomic DNA of PRV Bartha-K61 into Vero cells. After many cycles of blue plaguepurification and PCR identification,we got a PRV recombinant containing hemagglutinin (HA) gene ofH3N2 SIV and designated as rPRV -HA.
2.The hemagglutinin protein(HA) gene of avian influenza virus was amplified from pVAX1-HA by PCR and sub-cloned into pcDNA3.1(+) vector. HA-CpG gene was also cloned into the pcDNA3.1(+).
