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1.Methods Using human ALR cDNA sequence as a reference and BLAST path as a nucleotide homology search tool, GenBank has been searched for ALR homologous genomic DNA sequence.
方法采用人肝再生增强因子cDNA及其编码序列,对基因的核苷酸序列数据库(GenBank)以BLAST为工具进行核苷酸序列同源性比较分析,寻找相应的ALR基因组DNA序列。收藏指正
2.Objective To subclone a novel gene of Schistosoma japonicum(Sj), adenylate kinase (AK) cDNA, which was identified through expressed sequence tag (EST) strategy and homology search, so as to prepare for further functional study of this gene.
目的将用表达序列标签(expression sequence tag, EST)策略及同源性搜索发现的日本血吸虫新基因——腺苷酸激酶(adenylate kinase,AK)cDNA克隆到表达质粒pET32a(+)上,为下一步研究该基因的功能做准备。收藏指正
3.The cloned full length cDNA (csn), 804 bp in size, encoded a single peptide of 251 amino acids. The blast search results of the cDNA sequence showed that the sequence shared the homology with chitosanase of Fusarium solani in two region, specifically but showed no homology with known bacterial chitosanases. The two region have repeated aspartic acid (DIDCD) and threonin (TWTPT) amino acid residue, respectively.
用此序列推测的寡核苷酸引物扩增得到脱乙酰几丁质酶（csn）的cDNA，其全长为804bp，编码一种251个氨基酸的单肽，对csn的序列比较分析表明，它与茄病镰刀菌在两个区域具有同源性，这两个区域分别有重复天冬氨酸及苏氨酸残基，这说明它与细菌具有相同的演化起源。收藏指正
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