hormone assay
2.COMPARISON OF HISTOCHEMICAL AND DCC ASSAY FOR HORMONE RECEPTORS IN BREAST CANCER
3.(3) HKC which were co-stimulated with 10~ -7 mol/L ALDO and different concentrations of spironolactone or specific glucocorticoid hormone receptor inhibitor RU486 for 30min, then western blot assay was performed to detect the expression of phosphorylated and total ERK1/2 in the cell lysate of HKC.
4.The plasma prostaglandin E2(PGE2), adrenocorticotropin hormone (ACTH) and cortisol in experimental allergic encephalomyelitis (EAE) in guinea pigs was determined by ra-dioimmuno-assay.
5.AIM: To clarify the subtype of histamine (HA) receptor which mediates Adrenocorticotropic Hormone(ACTH) release from mouse anterior pituitary tumor cell line AtT-20. METHODS: ELISA assay was used to determine the effects of a variety of histamine receptor subtype-specific agonists and antagonists on ACTH release from AtT-20 cells.
7.Method:After the medicine treatment to assay serum estrogens (E2) content of the unmatured female rat by radioimmunelogic. At the same time ,assay the content of serum follicle hormone (FSH) and luteinizing hormone( LH)in OVX, compare the Jingxin Oral Liquid with grains for the influence of sex hormone whether have some difference.
9.Shaoxing duck is one of the famous Chinese local layer strains with small body size,early sexual maturation and excellent performance in egg production. In order to investigate the mechanisms of its reproduction,the serum levels of gonadotropin releasing hormone(GnRH)at 60,100,200 and 380 days(D)of age were determined using radio immuno assay(RIA),in comparison with Khaki Campbell duck counterpart.
10.Radioimmunoassay was used to assay the level of FSH（follicle stimulating hormone）, LH(luteinizing hormone), PRL(prolactin), E2（estradilo）, P(progesterone), T（testosterone）and β-EP(β-endogenous opioid peptides) in serum. At same time, the fluorospectrophotometry was used to analyze the level of 5-HT.
