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1.A PPV DNA C fragment obtainted by PstⅠ/Hind Ⅲ digestion and PUP recombinant plasmid formed by C fragment which was cloned to PUC 19 ,were labelled with nonradioactive Digoxigenin as DNA probes for Porcine Parvovirus(PPV) via molecule clone technology.
应用分子克隆技术制备的猪细小病毒复制型DNA的PstⅠ／HindⅢ双酶切片段C及被克隆到PUC19中的C片段形成的重组质粒PUP利用非放射性的地高辛标记后制备两种探针。收藏指正
2.Methods: PAF and PAF receptor antagonist BN52021 were administered through arachnoid space and intravenous injection respectively. The expression of intercellar adhesion molecule-1mRNA (ICAM-1mRNA) and endothelial cell leukocyte adhesion molecule-1mRNA(ELAM-1mRNA) in cervical cord vascular endothelial cells were measured by digoxigein labelled cDNA probe in situ hybridization technique after trauma.
方法：采用蛛网膜下腔注射PAF及静脉注射PAF受体拮抗剂BN52021，应用地高辛标记cDNA探针原位杂交技术检测颈髓损伤后颈髓血管内皮细胞细胞间粘附分子－1mRNA（ICAM－1mRNA）和内皮细胞白细胞粘附分子－1mRNA（ELAM－1mRNA）表达。收藏指正
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