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1.Methods The 30% TBSA Ⅲ deg r ee burned rats were subjected to fluid r esuscitation with rapid infusion (RI gro ups) and average infusion (AI groups). Th e myocardial microvascular permeability, the level of ET 1,NO in myocardium and t he l evel of myocardial myosin light chain 1(CMLC 1) in plasma were observed.
方法　采用大鼠 3 0 %TBSAⅢ度烧伤模型 ,动态观察烧伤延迟复苏快速补液和均匀补液后 ,大鼠心肌微血管通透性和心肌局部内皮素 1和一氧化氮等细胞因子含量及血浆中心肌肌球蛋白轻链 1浓度的改变。收藏指正
2.(2) 1μmol/L staurosporine completely inhibited 0. 5 U/ml thrombin,or 10μmol/L PMA or 20μmol/L A23187-induced the phosphorylation of two proteins，a 40kD substrate of protein kinase C (pleckstrin ), and the 20kD myosin light chain (MLC);
（2）1μmol／LStaurosporine几乎完全抑制0．5U／ml凝血酶、或10μmol／LPMA、或20μmol／LA23187诱导的血小板40kD和20kD蛋白磷酸化。收藏指正
3.differential screening and homology analysis identified 6 differentially expressed genes associated with cytoskeletal remodeling, including slingshot 2L(SSH-2L), PP1 catalytic subunit, beta isoform(PP1Cβ), dedicator of cytokinesis 8(DOCK-8), Homo sapiens protein tyrosine phosphatase non-receptor type 6 and 12(PTPN6, PTPN12), myosin regulatory light chain interacting protein(MIR).
结果:构建了高效的上调与下调消减cDNA文库,经鉴定及同源性分析有6个与细胞骨架重建有关,包括上调基因slingshot磷酸酶(SSH-2L),蛋白磷酸酶1β亚型(PP1Cβ),无功能糖基转移酶样蛋白(DOCK-8),蛋白酪氨酸磷酸酶非受体型6(PTPN6)与非受体型12(PTPN12);收藏指正
4.Results The sequence analysis demonstrated that the S cFv of V~{*-~}H and V~{*-~}L chains belong to heavy chain group V(A) and kappa light cha in group IV of mouse immunoglobulin variable region,respectively.
结果 :核苷酸序列分析证明 ,获得的单链抗体基因VH 基因属于小鼠抗体重链可变区基因家族的V(A)组 ,VL 基因属于小鼠抗体κ轻链可变区基因家族的IV组。收藏指正
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