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1.A METHOD OF INTRACELLULAR LUCIFER YELLOW INJECTION IN FIXED BRAIN SLICES COMBINED WITH RETROGRADE FLUORESCENT TRACING
荧光逆行追踪结合离体固定脑片细胞内染色技术收藏指正
2.Lucifer yellow iodoacetamide(LY) was used as a fluorescence marker to adriamycin which was encapsulated by magnetic thermosensitive liposomes.
利用LY荧光标记阿霉素后,制备成磁性热敏阿霉素脂质体。收藏指正
3.The function of gap junction intercellular communication(GJIC) of HTFs cells was detected by scrape-loading lucifer yellow dye transfer method.
染料传输法观察HTFs细胞间隙连接细胞间通讯(G JIC)的能力;收藏指正
4.Methods: Murine lung endothelial cells were subcultured and incubated with HMME for 24 hours. Then the cells were stained with Rhodamine 123 for mitochondrion? Lucifer yellow for lysosome?
方法 :传代培养鼠肺毛细血管内皮细胞 ,将血卟啉单甲醚 (HMME)与内皮细胞共同孵育 2 4小时后 ,加入线粒体探针Rhodamine 12 3、内质网探针DioC6 ( 3 )和溶酶体探针Luciferyellow分别对细胞器染色。收藏指正
5.Retinal ganglion cells were stained by Lucifer Yellow or biocytin which were injected into cells during whole cell recordings for the purpose of morphological study of RGCs.
全细胞记录的同时细胞内注入荧光黄或biocytin进行组织染色，观察RGCs的形态学特征。收藏指正
6.METHODS The isolated rat intestinal membranes were used to study the effects of Labrasol on the transportation of Rhodamine123(a subtrate of P-gp) and Lucifer yellow(non-subtrate of P-gp )by ussing chamber.
方法　用大鼠的离体肠段,通过体外Ussingchamber的转运实验,研究了Labrasol对P -糖蛋白底物若丹明1 2 3转运的影响,同时考察了非P -糖蛋白底物荧光黄在Labrasol作用下的转运行为。收藏指正
7.By Confocal Laser Scanning Microscopy(CLSM) and combined with fluorescence probe, 457Da Lucifer Yellow (LYCH)which was impermeable to the membrane, could enter the living parenchyma cell through ectodesmata-like. The result proved that ectodesmata-like still retained the function of the symplastic translocation.
借助激光共聚焦荧光显微镜并结合荧光标记物示踪，发现不透膜荧光物质分子量为457Da的Lucifer Yellow(LYCH)，可以经尝外连丝进入存活细胞内，由此确证了类外连丝的共质传输功能。收藏指正
8.METHODS Cell culture, flow cytometer (FCM) and Lucifer Yellow scrape loading and dye transfer (SLDT) were employed to detect the expression of Cx43 pro tein in HeLa and the regulatory effect on gap junctional intercellular communication treated by AT retinoic acid (ATRA).
方法　应用流式细胞仪以及 L ucifer Yellow划痕标记荧光传输技术 ,研究维甲酸作用对 He L a细胞 Cx43蛋白表达 ,以及细胞生长状态和间隙连接通讯功能的调节作用 .收藏指正
9.By using Northern hybridhation analysis, Cx43 immunofluorescent staining and Lucifer Yellow dye transfer methods,high level of Cx43 mRNA and protein expression was revealed in cultural nounal human elnbryothe lUng all. The Cx43 protein was locatal at regions of Cell inelnbrane and the induce of Cell contact comeponding to gap junctions. The function of gap junctional inteedtilar cornxnunication was shown by effident dye transfer.
用间隙连接蛋白Cx43cDNA探针Northern印迹杂交，Cx43抗体免疫荧光染色和罗氏黄荧光染料传输方法检查，正常人胚肺细胞的Cx43在mRNA和蛋白水平有高表达，Cx43蛋白免疫荧光分布在间隙连接的部位，细胞间隙连接通讯功能明显。收藏指正
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