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1.Conclusion: pED GCSF is a high eukaryotic expression plasmid in mammalian cell.
结论：pED－GCSF是一个能在哺乳动物细胞中高效表达rhG－CSF的真核表达质粒。收藏指正
2.I:Cell Aggregation Induced FGF8 Elevation is Essential for P19 Cell Neural Differentiation II:Visualization of bHLH Transcription Factor Interactions in Living Mammalian Cell Nuclei and Developing Chicken Neural Tube by FRET
I.FGF信号通路与P19细胞神经命运决定 II.荧光共振能量转移研究bHLH蛋白的相互作用收藏指正
3.Second, some reports utilized the baculovirus as a gene delivery vehicle into mammalian cells and did not have any bad effect on the growth of mammalian cell.
因此，本论文中讨论一些影响蛋白质表现量与延长表现的参数如:培养基的更换、重复感染、病毒感染方式。收藏指正
4.The mammalian cell expression vectors for Mac 1α subunit (CD11b) fused with blue fluorescent protein (BFP) at the carboxyl terminal or for Mac 1β subunit (CD18) fused with yellow fluorescent protein (YFP) at amino terminal were constructed to create recombinant plasmid of pCD11b BFP or pYFP CD18, respectively.
分别构建表达BFP与CD1 1b的C末端、YFP与CD1 8的N末端相连接的融合蛋白的表达载体 ,并将二者转染至既无内源性Mac 1的表达同时又具有某些炎症反应信号转导系统的CHO细胞株进行表达Mac 1 FP .收藏指正
5.ECL Western blotting results indicated that the target gene could express in the mammalian cell line NIH3T3. Conclusion Recombinant plasmid HSP27/pAAV-MCS had been cloned successfully, which would provide the foundation for investigating the role and the mechanism of HSP27 in the ischemia precondition of kidney.
重组质粒转染哺乳动物细胞系NIH3T3后,ECLWesternblot法证实了目的基因能在其中正确表达。 结论　成功地克隆真核表达重组质粒HSP2 7/ pAAV MCS ,为下一步研究HSP2 7对肾脏缺血预适应的作用及其机制打下了基础。收藏指正
6.METHODS:The pcDNA3.1(+)/ KGPcd was transfected into mammalian cell COS7 with Lipofectamine 2000.The transcription of pcDNA3.1(+)/ KGPcd was assayed by reverse transcription polymerase chain reaction (RT-PCR). The expression product of pcDNA3.1(+)/ KGPcd was detected by method of indirect immunofluorescent.
方法 :利用基因重组技术构建牙龈卟啉菌牙龈蛋白酶K基因重组质粒pcDNA3.1( + ) -KGPcd ,然后通过脂质体将 pcDNA3.1( + ) -KGPcd瞬时转染COS7细胞 ,以RT -PCR和间接免疫荧光法检测重组质粒的转录水平和蛋白表达情况。收藏指正
7.USA is the leader of biopharmaceutical industry, with 79 biotech drugs produced by genetic engineering, antibody technology or cytotechnology, being approved by FDA (18 products expressed by E. coli , 8 biotech drugs expressed by yeast, and 53 biomedicines produced by mammalian cell culture) , of which, 64 are recombinant therapeutic proteins.
美国FDA批准的以基因工程产品、抗体工程产品和细胞工程产品为主要代表的生物技术药物共79种(18种为大肠杆菌表达,8种为酵母表达,53种为哺乳动物细胞培养生产),其中基因重组蛋白质药物为64种。收藏指正
8.Methods According to China national standard GB /T16886docum ents,the toxicity o f Evergel prosthetic nucleus material was investigated by t he cytotoxicity test,sensitization test,haemoly sis test,Ames test,mice marr ow micronucleus test and chromosome aberration test of mammalian cell in vitro.
方法按照国家标准GB/T16886的要求,对Evergel人工髓核材料进行了包括细胞毒性试验、致敏试验、溶血试验、Ames试验、小鼠骨髓细胞微核试验和哺乳细胞体外染色体畸变试验在内的毒理学测试。收藏指正
9.The Luo Rudy models (LRd00) of mammalian ventricular cell were used in simulating of two dimensional (2 D) ventricular tissue.
用Luo Rudy心室肌细胞动作电位模型LRd0 0进行二维心室肌组织仿真研究。收藏指正
10.Study on the Function of Mammalian era in Cell Cycle
哺乳类era基因在细胞周期调控中作用的研究收藏指正
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