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1.Single nucleotide polymorphisms of INHBA gene were detected in both high fecundity sheep breeds (Small Tail Han sheep and Hu sheep) and low fecundity sheep breeds (Dorset sheep, Texel sheep and German Mutton Merino sheep) by PCR-SSCP.
本研究根据牛抑制素β_A亚基(inhibin β_A，INHBA)基因外显子1和外显子2序列设计7对引物，采用PCR-SSCP技术检测INHBA基因在高繁殖力绵羊品种(小尾寒羊、湖羊)以及低繁殖力绵羊品种(多赛特羊、特克塞尔羊、德国肉用美利奴羊)中的单核苷酸多态性，同时研究该基因对小尾寒羊高繁殖力的影响。收藏指正
2.The respective average number of lutein in ovary is Kazak sheep(1.192),Chinese Merino sheep(1.233) and Hu sheep(3.172).
哈萨克羊、新疆军垦细毛羊和湖羊卵巢上黄体生成的平均个数分别为1.192、1.233和3.172个。收藏指正
3.Any of a breed of merino sheep of French origin, raised for wool and meat.
兰布列羊一种产于法国的美利奴羊，为取毛或肉食而饲养收藏指正
4.In Hu sheep,German Mutton Merino sheep,Small Tail Han sheep,Suffolk sheep and Dorset sheep,the frequency of allele A was 0.672,0.786,0.846,0.857 and 0.867,respectively,and the frequency of allele B was 0.328,0.214,0.154,0.143,and 0.133,respectively.
统计结果表明:湖羊、德国肉用美利奴羊、小尾寒羊、萨福克羊和多赛特羊A等位基因频率分别为0.672、0.786、0.846、0.857和0.867,B等位基因频率分别为0.328、0.214、0.154、0.143和0.133。收藏指正
5.The Statistic results showed that in Hu sheep, German Mutton Merino sheep, Small Tail Han sheep, Suffolk sheep and Dorset sheep, frequency of A allele were 0.672, 0.786, 0.846, 0.857and 0.867 respectively, frequency of B allele was 0.328, 0.214, 0.154, 0.143, and 0.133 respectively.
统计结果表明:湖羊、德国肉用美利奴羊、小尾寒羊、萨福克羊和多奏特羊A等位基因频率分别为0.672、0.786、0.846、0.857和0.867,B等位基因频率分别为0.328、0.214、0.154、0.143和0.133。收藏指正
6.Single nucleotide polymorphism(SNP) of BMPR-IA gene was detected in both high fecundity sheep breeds (Small Tail Han and Hu sheep, respectively) and low fecundity sheep breeds (Dorset, Texel and German Mutton Merino sheep, respectively) by PCR-SSCP.
采用PCR-SSCP技术检测BMPR-IA基因在高繁殖力绵羊品种(小尾寒羊、湖羊)以及低繁殖力绵羊品种(多赛特羊、特克塞尔羊、德国肉用美利奴羊)中的单核苷酸多态性,同时研究该基因对小尾寒羊高繁殖力的影响。收藏指正
7.Bone morphogenetic protein receptor IA(BMPR-IA) gene was studied as a candidate gene for the prolificacy of sheep. Single nucleotide polymorphism of BMPR-IA gene was detected in both high fecundity breeds(Small Tailed Han sheep and Hu sheep) and low fecundity breeds(Dorset sheep,Texel sheep and German Mutton Merino sheep) by PCR-SSCP.
以骨形态发生蛋白受体IA(bone morphogenetic protein receptor IA,BMPR-IA)基因为候选基因,采用PCR-SSCP技术检测该基因在高繁殖力绵羊品种(小尾寒羊、湖羊)以及低繁殖力绵羊品种(多赛特羊、特克塞尔羊、德国肉用美利奴羊)中的单核苷酸多态性,同时研究该基因对小尾寒羊高繁殖力的影响。收藏指正
8.For primer 2-4, genotypes KK and KL were detected in German Mutton Merino sheep, three genotypes (KK, KL and LL) were detected in other four sheep breeds.
引物2-4,德国肉用美利奴羊只出现KK和KL基因型,而其余4个绵羊品种则出现KK、KL和LL基因型;收藏指正
9.For primer 2-2, three genotypes (GG, GH and HH) were detected in Dorset and German Mutton Merino sheep, genotype GG was detected in other three sheep breeds.
引物2-2,多赛特羊和德国肉用美利奴羊出现GG、GH和HH基因型,而其余3个绵羊品种只出现GG基因型;收藏指正
10.The homology analysis of the GH gene of sequences by Clustal W software indicated that the nucleotide sequence homologies of GH gene of Boer goats were 98.5% with Tokara goats,97.0% with Merino sheep,and 95.4% with bovine GH genes;
应用ClustalW软件进行同源性分析,结果表明,波尔山羊GH基因与Tokara山羊、澳洲美利奴羊和黄牛的GH基因的相似度分别为98.5%,97.0%和95.4%;收藏指正
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