messenger DNA
1.Antisense nucleic acid technique is a method using single-stranded complementary sequences with specificity assigned by Watson-Crick base-pairing targeted specific messenger RNA or DNA to block expression of target gene.
3.A DNA molecule to which RNA polymerase binds, initiating the transcription of messenger RNA.
4.convert the genetic information in (a strand of DNA) into a strand of RNA, esp. messenger RNA; in biochemistry.
5.The deoxyribonucleic acid (DNA) binding activities of NF κB, the inhibitory kappa B (IκBα) levels in cytoplasm and tumor necrosis factor α (TNF α) messenger ribonucleic acid (mRNA) expressions were determined after 5, 15 min ischemia in experimental group, both after 0, 5, 15, 30 min ischemia and concomitantly 5, 15, 30, 45, 60 min reperfusion in control group.
6.Methods Total RNA of 24 new born bovine trabecular tissue was extracted by guanidine isothiocyanate method. The TGF β 33 plasmid was brought into colibacillus HB101 and amplified. After Bam HI enzyme degradation and label with α 32 P dATP, the RNA was hybridized with the complementary DNA (cDNA) probe and examined by autoradiography to determine the expression of messenger RNA (mRNA) for TGF β 1. Results The presence of mRNA for TGF β 1 in bovine trabecular meshwork was confirmed.
