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1.A novel anti-Tobacco mosaic virus(TMV) protein designated as gynostemmin was isolated from Gynostemma pentaphyllum. Its molecular mass was 27 kD and the N-terminal 19 amino acid residues were DINFSLAGADGQTYNTFIA(SWISS-PROT accession number P83206).
以半叶法测定抗烟草花叶病毒(Tobaccomosaicvirus,TMV)活性为监控手段,采用(NH4)2SO4分级沉淀、阳离子交换及亲和层析等方法,从绞股蓝(Gynostemmapentaphyllum)中分离到一种抗TMV蛋白,其分子量约为27kD,N端19个氨基酸残基为DINFSLAGADGQTYNTFIA(SWISS-PROT登录号为P83206)。收藏指正
2.STUDIES ON COAT PROTEIN OF XINJIANG WATERMELON MOSAIC VIRUS-2
新疆西瓜花叶病毒2号外壳蛋白的研究收藏指正
3.The Analysis on the Characteristics of the Coat Protein Genes of Cucumber mosaic Virus Isolated from Echinacea purpurea Moench
黄瓜花叶病毒紫松果菊分离物外壳蛋白特性分析收藏指正
4.Sixteen isolates of cucumber mosaic virus (CMV) from China, were divided into two main serogroups by protein A sandwich enzyme linked immunosorbent assay (PAS ELISA) in comparison with the four reference strains, Fny, Lny, M and WL. Fourteen isolates and Fny, M strains belonged to DTL serogroup, two isolates and Lny, WL strains to ToRS serogroup.
根据A蛋白夹心酶联免疫吸附法（PAS－ELISA）试验结果可以将我国分离的16个黄瓜花叶病毒（CMV）分离物及4个CMV标准毒株（Fny、Lny、M、WL）区分为2个血清组．14个分离物及标准毒株Fny、M属DTL血清组，2个分离物及标准毒株Lny、WL属ToRS血清组收藏指正
5.It has been identified as a corider isolate of cucumber mosaic virus (CMV-Co) on the basis of its host rang, transmission, stability, particle morphology, serology, ultraviolet absorption spectrum and molecular weight of coat protein subunit This virus could infect 21 plant species belong to 5 families by sap inoculation.
花叶病株上分离到一病毒分离物(CMV-Co),汁液摩擦接种和桃蚜(Myzus Persicea)均能传毒。 人工摩擦接种可侵染5科21种植物,在黄瓜、心叶烟等植物上引起系统花叶;收藏指正
6.Based on the coat protein (CP) gene sequence (480nt)of Tobacco mosaic virus (TMV), a 442bp fragment was amplified by reverse transcription polymerase chain reaction (RT-PCR) with the specific primers.
依据烟草花叶病毒(Tobacco mosaic virus，TMV)外壳蛋白(CP)基因序列(480nt)片段设计合成了一对引物，以病毒粗提RNA为模板，通过反转录—聚合链式反应(reverse transcription polymerase chain reaction，RT-PCR)扩增得到长度为442bp的目的片段。收藏指正
7.The loading dynamics of viral genomic RNA1,RNA2,RNA3 and coat protein(CP) of Cucumber mosaic virus CNA strain(CMV-CNA) in systemic tissue of infected tomato seedlings under 22-26℃,as well as the symptom severity of host plants was investigated.
采用实时荧光定量PCR(FQ-PCR)和DAS-ELISA方法,研究了22~26℃温室条件下番茄幼苗中黄瓜花叶病毒CNA株系(CMV-CNA)各基因组RNA组分及其外壳蛋白(CP)含量的动态变化,同时结合同期感病植株症状发展和病情指数,分析并探讨CMV各基因组RNA、CP以及病症显示程度之间的时间效应及其相关性。收藏指正
8.Tobacco and capsicum were treated with 30μg/mL Harpin protein by spraying over the plant, and then Tobacco mosaic virus (TMV) was inoculated in the plants by mechanical inoculation after 5 days. The TMV concentration in leaves was determined by ELISA.
将烟草和辣椒用30μg/mL Harpin喷雾处理5d后,接种烟草花叶病毒(Tobacco mosaic virus,TMV),观察植株病症,并采用ELISA检测TMV含量。收藏指正
9.Two genes, the replicase gene from Banana bunchy top virus and coat protein gene from Cucumber mosaic virus, were firstly fused together with PCR, and were inserted into a plant expression vector-pBI121. The plant expression vector was transformed to Agrobacterium tumefaciens LBA4404. As a result, the engineered agrobacteria containing pBI121.FBC were gotten, and would be further used to develop a engineering banana variety resistant to two kinds of viruses.
利用重组PCR技术将香蕉束顶病毒的复制酶基因和黄瓜花叶病毒的衣壳蛋白基因融合重组,并与植物表达载体pBI121连接,构建了抗香蕉束顶病和香蕉花叶病的双价植物表达载体,通过冻融法转化载体入根癌农杆菌LBA4404,获得了工程农杆菌pBI121.FBC,从而为培育抗病转基因香蕉品种奠定了基础.收藏指正
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