mut
2.Mut-HPI was less easily digested by trypsin and CPB than Met-HPI.
3.Two mutants,named mut-1 and mut-2,with decreased ADH activity were screened out by yeast peptone dextrose(YPD)agar medium containing allyl alcohol. These two mutants had decreased ADH activities of 41.63% and 50.29% compared with the parent strain.
4.SDS-PAGE results indicated that there was a clear target band in Muts and Mut+ recombinant Culture supernatant after 48 hours culture respectively.
5.Mut für die Zukunft: Analyse der Rede von Bundespr?sident Horst K?hler bei der Gedenkveranstaltung zum 60. Jahrestag des Endes des Zweiten Weltkrieges in Europa.
6.SDS-PAGE results showed that there was a clear target protein band in Mut+ recombinant supernatant after 48 hours of culturing, while a faint band only in Muts recombinant after 72 hours.
7.Methods Site-directed mutagenesis was conducted to obtain 2 mutant human XIII A recombinant plasmids, mut-PCI/FXIIIA. Normal wild type factor XIII A recombinant plasmid, wt-PCI/FXIIIA, and mut-PCI/FXIIIA, were transfected into cultured COS7 cells line, renal fibroid cell of African green monkey using Superfect reagent respectively, The expression levels of DNA, RNA and protein of human factor XIII, both wild type and mutant, were detected by PCR, RT-PCR and Western blotting.
8.2、 Construction of luciferase reporter plasmid containing human a-SMA genepromote (p895-Luc) and mutant Smad2 plasmid (pSmad2~(mut)): The human a-SMAgene promoter (-895~+9bp) was cloned by PCR from human genomic DNA andwas inserted into vector pGa(?) 3-basic at MluI-XhoI site and form p895-Luc.
9.An increase of intracellular DNR retention in si-MDR1-treated cells was observed, confirming the inhibition of P-gp function by siRNA targeted MDR1. One base pare mutated control (si-MDR1-Mut) lost the effect of si-MDR1 on both the degradation of mdr1 mRNA and the reduction of P-gp expression.
