nucleic acid sequence
7.After cloned and sequenced,the sequence of full-length cDNA of FRG4 gene which rapid isolated using hot - start PCR technique was analyzed for multiple sequence alignment and showed that FRG4 has highly homogeneity with Homo sapiens Synapse - associated Protein, SAP1 (with nucleic acid homogeneity 99% and amino acid homogeneity 87%).
8.etection of citrus exocortis viroid(CEV)was conducted by using photobiotin -labelledF20 probe. Dot hybridization indicated hat F20 was very specific to CEV sequence, and thatthe highest dilution of crude CEV-nucleic acid extracts and the minimal amount of CEVdetected was about 1/100 and 1ng rtspectively.

