2.People has developed some kinds of mimics of oligonucleotides , among them there is PeptideNucleicAcid(PNA), which was first synthesized by scientists at the University of Copenhagen(Nielsen, Egholm et al).
3.objective To improve the detection sensitivity of peptidenucleicacid (PNA) piezoelectric gene sensor by introducing "RecA protein-complementary single strand DNA probe" complex as new style biological signal amplification system.
4.Results Antisense peptidenucleicacid (PNA) targeting the coding region of the bcl 2 messenger RNA can effectively inhibit HL 60,K562 cells viability ,down regulate the synthesis of bcl 2 protein and increase cell apoptosis.
