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1.perfect binding; threadless binding;
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2.Results Sensitivity of method was 0.22ng/ml, Recovery rate 97.3～102.6%(average 99.8%) the average rate of nonspecific binding (NSB) was 3.6%,the coefficient of variation of within-run was 5.4% (average) between-run was 8.2%(average) perfect experiment result was good.
结果本法灵敏度为0．22ng％，回收率97．3％～102．6％，平均回收率99．8％，非特异性结合率（NSB）平均为3．6％，批内平均变异系数CV5．4％，批间平均变异系数CV8．2％，健全性实验结果良好。收藏指正
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