plasma clot explant culture
2.Experimental studies demonstrated that the produced erythroid colonies (E-CFU-D) after 2 days of culture in plasma clot diffusion chamber were neither derived from ERC nor from immature erythroid cells (pronormoblast, early normoblast and intermediate normoblast), but from a subpopulation of erythroid progenitor cells which is in the differentiation pathway between ERC and pronormoblast.
4.[Methods] 20 placental samples of pregnant women with ICP and 20 normal term placental samples were investigated the expression of HIF-1α and -2α mRNA by RT-PCR. Protein from villous explant culture under hypoxia condition (n=8each) was probed for HIF-1α, HIF-2α and P53 by immuno-histochemistry methods.
5.Result shew: the number of chondrocyte of enzyme digestion was more than that of explant culture,The viability of chondrocytes isolated with collagenase typeⅡconfected by serumfree medium were markedly higher than that of PBS (free of Ca~ 2+ ,Mg~ 2+ ). There was statistical discrepancy between the two enzyme digestion groups(P<0.05).
6.The IgG of a monoclonal antibody N34 specific for urokinase and the Fab fragment of a monoclonal antibody specific for the granule membrane protein GMP-140 of activated platelet were chemically cross linked by disulfide bond. The coupled bispecific antibody(N34-SZ-51) was able to bind urokinase and plasma clot simultaneously.
