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1.Using Ri-plasmid to Transfer Tomato Primarily
Ri质粒转化番茄的初步研究收藏指正
2.Methods: hEn gene was from plasmid of pCA13-hEn and cloned into shuttle plasmid and formed transfer plasmid of pAdtrack-cmv-hEn. After linealized with PmeⅠ and co-transformed into BJ5183 bacterial cells with adenovirus genomic DNA plasmid of pAdeasy. The identified recombinant adenovirus plasmid DNA was digested with PacⅠand transfected to 293 cells for package of recombinant adenovirus particles.
方法 :将hEn基因自 pCA13-hEn质粒中酶切下来 ,亚克隆至腺病毒穿梭质粒中 ,形成转移质粒 pAdtrack -CMV -hEn ,并用PmeⅠ线性化后与腺病毒基因组质粒 pAdeasy共转化大肠杆菌BJ5 183,抽提重组体腺病毒基因组质粒DNA ,PacⅠ酶切后在 2 93细胞中包装成腺病毒颗粒。收藏指正
3.Effective transfer of indigenous plasmids from R. leguminosarum biovar phaseoli strain RCR3622 was accomplished by transposon Tn5-Mob accompanied by the mobilizing plasmid RP4-4 Transfer of two of the four plasmids of RCR3622 into R.
转座子Tn5-Mob在质粒RP4-4配合下能诱动(Mobilization)菜豆根瘤菌RCR3622内源质粒的诱动转移。 在种间根瘤菌杂交过程中,二个巨型质粒的转移频率均大于10~(-3);收藏指正
4.Methods:GIP-hIns gene was cut from plasmid of pCDNA3-GIP-hIns and cloned into plasmid pCA13 - ACMV, which the plasmid pCA13 with the CMV promoter deletion and formed transfer plasmid of pCA13-GIP-hIns. The plasmid was co-transfected to 293 cells with plasmid pBHGE3 for package of recombinant adenovirus.
方法:将GIP- hIns基因自pCDNA3-GIP-hIns质粒中酶切下来,克隆至切除CMV启动子的pCA13-△CMV质粒中,形成转移质粒 pCA13-GIP-hIns,然后与质粒pBHGE3共转染至293细胞株,在其中同源重组形成腺病毒颗粒。收藏指正
5.Study on The Transfer of the RP4 Plasmid to Y.pestis
RP_4质粒转移给鼠疫菌的研究收藏指正
6.Conjugation Transfer of Plasmid pMC73A from K. Oxytoca to Acacia Rhizobium
质粒pMC73A从催娩克氏菌到相思根瘤菌的接合转移收藏指正
7.Methods HeNOS cDNA was digested from plasmid pHCMV SP1A-heNOS with EcoR I and subcloned into the shuttle vector to generate the transfer plasmid pShuttle-heNOS-EGFP.
方法:质粒载体pHCMV SP1A-heNOS用EcoRⅠ酶切,回收heNOS cDNA,亚克隆至腺病毒穿梭质粒中,形成转移质粒pShuttle-heNOS-EGFP;收藏指正
8.The experimental result showed th t lower pH,phosphate starvation and acetosyringone could activate the expression of the vir genes of Ti-plasmid in Agrobacterium tumefaciens and promote T-DNA transfer into mung bean cells to some extent through transient expression of GUS activity in mung bean cells.
根癌农杆菌经乙酰丁香酮等诱导物质以及低pH、低PO预培养后，细菌T－DNA的转移受到促进，GUS基因表达活性明显提高。收藏指正
9.STUDY ON CONJUGATION AND TRANSFER OF COL PLASMID HARBORING IN FIVE SPECIES OF E. COLI
5种大肠埃希氏菌Col质粒接合传递实验研究收藏指正
10.3) Plasmid pMC73A can be transferred from Koxytoca NG13 to Acacia Rhizobium MZ by conjugation, and the transfer frequency are 8.0×10~(-8).
3、运用接合转移的方法把质粒pMC73A从供体菌催娩克斯菌NG13引入受体菌相思根瘤菌MZ和AJ018中，接合频率都为10~(-8)数量级。收藏指正
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