plate assay
2.DETERMINATION OF THE ANTIMICROBIAL ACTIVITY OF PROBIOTIC BY OXFORD PLATE ASSAY SYSTEM
5.Conclusion Micro-plate Hybrid Capture Detection PCR Assys is a rapid,easy,sensitive and special assay to detect high-risk HPV infection with cervical cytological specimens,it can be applied for large-scale screening and clinical diagnosis.
6.Using a self prepared coated ELISA plate and anti dsDNA quantitative kit of Binding site company of Birmingham medical college U K,and dot immunogold filtration assay have detedted sera of 32 patients with active SLE,6 rheumatoid arthritis and 30 normal controls,results showed,positive consistent rate is respectively 96 9% and 93 8% and negative consistent rate is 96 7%,5 patients with SLE were tested with the detection of quantitative,results were almost consistent.
7.A Sandwich Enzyme-linked immunosobent assay test Differentiability kit based on previous work was developed for detection of avian influenza virus (AIV) antigen including all AIV subtypes detecting, subtype of ATV H5 and subtype of AIV H9. The kit consists of twelve reagents and a piece of 96 well microtiter plate.
8.The capsular polysaccharide ( Cps ) of App was extracted and used as antigen for developing a Cps-based indirect enzyme-linked immunosorbent assay ( Cps-ELISA ) . The conditions of Cps-ELISA were determined as follows: 3μg/ml of CPS used to coat ELISA plate, 1:100 of sera as detecting samples and serum sample being incubatedat 37℃ for 90 minutes.
9.By using yeast two hybrid system, interaction assay in vitro between ubiquitin and IAP2 or IAP3 of SeMNPV has been done. The putative positive clones only existed in the plate of the SD/-Leu/-Trp/X-α-GAL, which suggested that ubiquitin might interact with IAP2 or IAP3 weakly, since ubiquitin may be transferred to IAP2 or IAP3 through E1 and E2 in yeast.
10.Methods The cultured human umbilical vein endothelial cells (HUVEC) and bovine vascular smooth muscle cells (VSMC)were treated with 0, 1.25, 2.5, 5.0, 10, 20, 40 Gy32P beta irradiation, respectively. The proliferation of the cells was then measured using colorimetric assay and analyzed with an ELBA plate reader. The erasion trace test was performed to discern the cellular inhibition of HUVEC and VSMC proliferation.
