pregnant female
5.Methods One hundred and forty pregnant Kunming female mice were randomly assigned into 14 groups(n=10 for each group). DES,17α-E2 and 17β-E2 were subcutaneously administered at a dose of 25,50,100,200 μg/(kg·d)from gestational day(GD)9 through GD17.The control group and normal control group were given dimethyl sulfoxide(DMSO)and saline alone,respectively.
7.To determine whether the level of nitric oxide (NO) in hypothalamic supraoptic nucleus (SON) and paraventricular nucleus (PVN) is related to reproduction, the changes of NOS neurons in SON and PVN of pregnant, lactating and virgin female rats were studied by using NADPH diaphorase histochemistry and NOS immunohistochemistry.

