primary marrow
7.The results indicated that the clinical features of this case were complicated and multiplex, the diagnosis was confirmed by using bone marrow smear and biopsy, immunophenotype analysis, conventional cytogenttics and fluorescence in situ hybridization (FISH), the prognosis of patients improved by active treatment for primary disease.
8.Results1 When other factors are the same, the density gradient isolation is better than whole-marrow isolation ,2.5X105/cm2 is the best plating density, fist medium changing at the fifth day is the best time at primary culture, DMEM medium is better than a-MEM, serum C is the best serum of the four serums compared, 10% is the suitable serum concentration.

