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1.The generation interval of current breeding scheme is 4.48 years, ratio of input and output is 1:14.04, the number of ram progeny test is low, and the useful life of ram and ewe is longer and so on can be improved.
现行方案世代间隔为4.48年,投入产出比为1:14.04,公羊后裔测定的后代数低,种公母羊的利用年限较长,还有较大的改进余地。收藏指正
2.In the subsequent generation, these inserts could segregate away from each other, allowing the selection of the progeny plants with only the Xa21.Using the DRB binary vector system, We abtained 41 transgenic plants , including japonica restorer line C418 and indica restorer line shuhui 527, chuanghui 11 .PCR and Southern blot analysis revealed that forein gene were intergrated into the genome of these plants.
11棵创恢中有7棵Xa21基因PCR阳性,3棵蜀恢527全为Xa21基因PCR阳性. 通过转基因后代自交分离,有5个植株的C418得到无标记基因的后代,能够得到无标记基因转基因植株占总转基因的收藏指正
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