protein engineering
3.Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases.
4.Improvement in thermostability of enzymes by protein engineering may introduce Pro at suitable β|turns and random coils. The rigid pyrrolidine ring can make the conformation of surroundings to be more reasonable by reducing the backbone unfolding entropy of proteins.
7.Although there are a lot of facts that limited the plants acting as bioreactor of the medical protein, the advantages and disadvantages coexist at present, it is a new development tendency in using transgenic plants to produce medical protein in plant gene engineering study.
8.The successful cloning and expressing of vp 1 gene become thebasis for the immunological studies of VP1 protein and themanufacture of genetic engineering vaccine of CAV.
9.AIM:To express the protein encoded by bone morphogenetic protein(BMP) 2 induced gene 3 kb(BIG 3) by using the gene engineering techniques.
10.Objective:To clone latent infection membrane protein 2A(LMP2A) cDNA of Epstein Barr virus and study its genetic engineering vaccine.
