protein equilibrium
1.Plasma protein binding of ~3H-dauricine was studied with an equilibrium dialysis technique; and the bindings of ~3H-dauricinewere 81. 44±1. 00% in blood bank plasma, 77.80±7.42% in plasma ofhealthy volunteer subjects and 75. 25±9.59% in plasma of rabbits. The dif-ferences between them were not significant (P>0. 05).
2.Dimeric AK was used as a model of oligomeric protein to investigate itsunfolding/refolding pathway in thermal or denaturant denaturation, with theaim to trap the possible equilibrium or kinetic intermediates.
3.It was noted that the binding between NE2 protein and 8H3 can be enhanced by the pre-binding of 8C11. The equilibrium dissociation constants (KD) of these three MAbs and their Fab fragments were determined by BIAcore biosensor.
4.The method of Burton was used to identify the DNA content in the nuclei. The amount of the receptor of cytoplasm was expressed by fmol/mg protein and the receptor of nuclei was expressed by fmol/mg DNA. Scatchard plot analysis was made, the Kd (equilibrium dissociation constant) of 17β-estradiol is about-10-10M.
