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1.Permeability of BBB was determined by extravasation of the protein-bound Evans blue dye to cerebral cortex and further evaluated by immunohistochemical or electronmicroscopic method.
BBB的通透性以结合有伊文氏蓝染料的血浆蛋白外渗进入脑组织的量以及免疫组化和电镜方法进行了测定。收藏指正
2.And purified soluble linoleate isomerase was gained by refolding of denatured LI protein bound to Ni2+-NTA, and the purified recombinant linoleate isomerase possessed biological activity(5.12 U/mg),which was 2.5 times of natural linoleate isomerase.
采用Ni2+-NTA柱上复性法获得有活性的纯化重组亚油酸异构酶,其比活力5.12U/mg,是天然亚油酸异构酶比活力的2.5倍。收藏指正
3.Methods:After homocysteine was liberated from its protein bound by tri n butylphosphine,all free form homocysteine were precolumn derivatized with 7 fluorobenzo 2 oxa 1,3 diazole 4 sulfonamide(ABD F)and eluted with 0.15 mol/L phosphate buffer(pH=4.2)containing 15% methanol. The analyses,carried out on a reversed phase C 18 column,were based on spectrofluorimetric detection.
方法 :血中结合态的HCY经三丁基膦还原为游离HCY后 ,与衍生试剂 7 氟苯唑 2 氧 1,3 二唑 4 磺酸胺 (ABD F)进行柱前衍生反应 ,衍生物经反相C18色谱柱 ,用 0 .15mol/L磷酸盐缓冲液 (pH =4.2 )与甲醇按 85∶15配制的混合液洗脱。收藏指正
4.The results of electrophoretic mobility shift assay (EMSA) indicated that GmDREB5 protein specifically bound to DRE (Dehydration-Responsive Element) and GmERF1 protein specifically bound to ERE (Ethylene-Responsive element or GCC-box element) and DRE in vitro;
本研究通过凝胶阻滞实验证明GmDREB5蛋白在体外能够与DRE顺式元件特异性结合,GmERF1蛋白在体外能够分别与ERE(GCC元件)和DRE顺式元件特异性结合;收藏指正
5.The binding of the 64kd protein with 3H-adenosine was blocked much more strongly by the adenosine-transport inhibitore dipyridamole and NBMPR than by adenosine receptor agonists NECA and R-PIA, which indicated that the protein is the membrane-bound adenosine transporter inbovine brain.
腺苷转运体抑制剂潘生丁和NBMPR对64kd蛋白与3H－腺苷的结合抑制作用远强于腺苷受体的激动剂NECA和R－PIA； 这表明64kd蛋白为牛脑细胞膜上结合的腺苷转运体。收藏指正
6.The SVMProt program is also used to identify the function of 3 SARS proteins-E protein, N protein and ORF13. E protein is predicted to be a membrane-bound protein and N protein a RNA-binding protein. Both of these predicted results are in agreement with existing knowledge.
应用SVMProt对SARS冠状病毒的3个蛋白质（E protein, N protein和ORF13）进行了预测，结果显示：E protein是一种能与膜蛋白结合的蛋白质，N protein是一种能与RNA结合在一起的蛋白质，两结果与实际事实完全吻合。收藏指正
7.When a protein is desiccated in the presence of trehalose, water molecules bound to the protein structure by hydrogen bonding evaporate and the sites are then replaced by the sugar to maintain an intact confirmation (42?45).
当蛋白在海藻糖存在下干燥，经氢键结合在蛋白结构上的水分子蒸发掉，留下的位点随后被糖替代，确保了结构的完整。收藏指正
8.On m2AchR-Gila fusion protein, Ach and Cch induce a 1 arge amount of bound [35 S ] GTPS whereas Atropine and AF-116 do a bit.
各种配体使单一的mZAchR蛋白与〔” 51 GTp，S的结合量无明显差别，结果表明叭AchR一Gila融合蛋白中的n认chR保留了与特异性配体结合的特性，以及融合蛋白两组分间确切地发生了偶联作用。收藏指正
9.Antibody against β-bungarotoxin bound protein represented presynaptic membrane antibody(PrMab),that against α-bungarotoxin bund protein belonges to acetylcholine receptor(AchR)antibody.
凡与β-BGT板结合蛋白起反应者为抗突触前膜抗体,凡与α-BGT板结合蛋白起反应者为抗AchR抗体。收藏指正
10.Purified ATF-lys10 specifically bound to uPAR-expressing cells and formed protein-DNA complexes with plasmid pGL3-control.
将uPAR反义核酸表达质粒与ATF一lyslo以及多聚赖氨酸组装成基因转移复合物，并转染95D细胞。收藏指正
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