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1.In chapter two, for research of the relatedness of EFPs of different group NPV, two recombinant baculoviruses were constructed via Bac-to-Bac system. HaSNPVgp64+egfp+ possessing GP64 of AcMNPV and EGFP, and HaSNPVegfp+ with an EGFP as control. Western blot analysis indicated that GP64 was expressed in HzAMI cells infected with HaSNPVgp64+egfp+ and was packaged into progeny BVs.
第二章 利用Bac-to-Bac系统,构建了带有AcMNPV膜融合蛋白GP64的重组病毒HaSNPVgp64+egfp+和对照病毒HaSNPVegfp+,Western blot分析表明,证明GP64可在HaSNPVgp64+egfp+感染的HzAM1中表达,并被成功地包装入子代BV。收藏指正
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