recombinat
1.Experimental Study of the Effect of Recombinat Human Interleukin-6 on the Growth and Metastasis of BTT_(739) Mouse Bladder Carcinoma
2.Conclusion These results suggest that the recombinat human interleukin-6 has a strong inhibitory metastasis of BTT 739 mouse bladder carcinoma.
3.Objective To study the inhibition effect of recombinat human interleukin-6 on the growth and metastasis of BTT 739 mouse bladder carcinoma.
4.colt JM109 cells. The recombinat cells, which were stable and genetic, were obtained. After 100 generations, the number of cells containing plasmid pZZ1 was more than 90%.
5.coli fusion expression pTO-T7 vector which allows the overexpression of a target protein. The recombinat plasmid pTO-T7-apcA was transformed into E. coli BL21(DE3) and induced in 0.5mmol/L IPTG in 37℃,the induce product was identified by western blotting and mass spectrum.
6.Methods Serum specimens from 266 children of different ages were tested by an indirect enzyme-linked immunosorbent assay (ELISA) for specific IgG against recombinant Norwalk virus particles (rNV), recombinant Mexico virus partides(rMX) and recombinat 387 Virus particles(r387), respectively, and fecal specimens were collected for detection of HuCV RNA using reverse transcriptional (RT)-PCR assisted by sequence analysis on the basis of comparison of the amplified product with the sequence entries in GenBank.
7.We investigated the effect of cell culture condition, such as seeding density and interval of replacing medium. Osteoblasts were induced and differentiated by cultivation ofconfluent cells in the presence of osteogenic supplement (containing 10~7mol/l dexamethasone (Dex), 50mg/l ascorbic acid, and 10mmol/l beta-glycerophosphate) or 100 ng/ml recombinat human bone morphogenetic protein-2(rhBMP-2).
8.It indicates MDRV is possible to be a new revirus that different from ARV.The DNA fragment of σC was subcloned into prokaryotic expression vector pET32a and the specific fusion protein with molecular weight 50 ku was expressed. Western blotting assay indicated that the polyclonal antibody against DRV could recognize the recombinat σC protein. It also provided technical support for developing an advanced gene engineering vaccine against muscovy duck reovirus.
