2.177 coding DNA sequences (CDS) were used to analyze the relative frequency of synonymous codon (RFSC), and 7 high-frequency codons including TAA, GCT, GAT, CTT, AGG, AGA and GTT were revealed by high-frequency codon analysis.
3.manB48, manB49 and manB36 have been registered in Genbank under the accession number of AY907668, AY913796 and DQ351940. The highest homology of manB48 and manB49 with other genes coding P-mannanase are 62.3% and 62.4% whereas that of DQ351940 is relative high.