restriction digest
1.Results: hIL-2 DNA frag- ment from pLXSN with EcoRI-BamH Ⅰ restriction enzymes digest was successfully cloned to pBluescript ⅡSK+/-.
2.Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence (ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment.
3.PCR-RFLPs of the ITS region of 11 intra-populations of soybean cyst forming nematodes were made with five restriction enzymes. Four of which, Alu I, Cfo I , Rsa I and Mva I , produced the same digest pattern among all the 11 populations.
4.Methods Digest plasmid pSFV-MCS with restriction endonuclease,and insert the obtained SFV replicon into vector pIRESneo containing CMV promoter and enhancer to construct a novel eukaryotic expression vector pCS. Insert HIV-1 MEGp24 gene into vector pCS,then transfect the constructed recombinant DNA vaccine pCS-MEGp24 to BHK-21 cells in mediation of liposome.
