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1.So we develop such study as follow:Experiment One Study on Reversal Effect of GLM Chloroform Distill Substance-MYJ1 on MDR Tumor Cells[Aim] To know whether Chinese GLM can reverse tumor cells MDR. [Methods]1. MTT assay 、 trypanblue dyeing assay determine cytotoxity of MYJ1.2. Accummulation and exclusivation experiment of Rhodamine determine the function of P-glyciprotein( P-gp) on membrane of tumor cells.
结论:敏药碱具有较强的体外逆转肿瘤MDR的活性,其逆转机制为通过抑制MDR肿瘤细胞膜P-gp功能,增加MDR肿瘤细胞内抗癌药物的累积而实现逆转肿瘤MDR作用,而且具有体内抗肿瘤活性及增效作用。收藏指正
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