2.Methods:After being routinely embedded in paraffin,complete serialsections of right atria were cut and stained with HE,Masson trichrome and with phosphotungstic acid hematoxylin,10 cases were observed under light microscope.
3.The cytoarchitectural organization of medial region(Mo),andinternal(L_1),middle(L_2),and external(L_3)zone of lateral region in theventrolateral part of the midbrain periaqueductal gray were studied in cresyI-violetstained serialsections from 13 rats applying morpho-quantitative analysis.
5.Serial paraffin sections were made from every tissue and and stained as follows: (1)hematoxylin and eosin stain, (2) immunostaining for Hp, and (3) double-staining using human gastric mucin (M1) immunostaining and alcian blue (AB).