selective disadvantage
1.AOAC, FDA and China are using conventional detection methods for Listeria monocytogenes include selective enrichment, isolation of colonies on agar and confirmation using hemolysin activity and other biochemical reaction as diagnostic tests. These methods are laborious and it takes 4-7 days to achieve a confirmed identification, which can not adapt for the need of rapid, specific and sensitive detection and disadvantage for food administration and disease prevention.

