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1.AOAC, FDA and China are using conventional detection methods for Listeria monocytogenes include selective enrichment, isolation of colonies on agar and confirmation using hemolysin activity and other biochemical reaction as diagnostic tests. These methods are laborious and it takes 4-7 days to achieve a confirmed identification, which can not adapt for the need of rapid, specific and sensitive detection and disadvantage for food administration and disease prevention.
目前AOAC、美国FDA和我国都采用传统的分离培养、生化鉴定的方法检测LM,这种方法检测时间为4~7天,检出限为10~4cfu/ml,费时费力,不能满足食品中致病菌及时、快速、灵敏的检测需要,不利于食品监督管理和疾病预防,因此,建立一种快速、特异、灵敏的LM检测方法迫在眉睫。收藏指正
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