sense cell
1.To study the roles of cyclin D3 in growth of leukemic cells and leukemogenesis, a T-cell leukemia cell line CEM which expresses cyclin D3 at a high leve was selected. With flow cytometry, the effect of cyclin D3 anti-sense oligodeoxynucleotides on cell growth and the expression of cyclin D3 at protein level in CEM cells was investigated.
3.habition ofSmac exPression with ani-sense smac could facilitate the cell growth and Gl/G0phase cells would be increased, which the sensihvity to chemotheraPy drug decreased.
6.(2) Eukaryotic expression vectors of sense and antisense SLP-2 were constructed and transfected into HEC-1B cell line using lipofectamine respectively. The morphological changes of the cell was observed by phase contrast microscopy. The cell growth was detected by methyl thiazolyl tetrazolium (MTT) assay, and the cell cycle were analyzed by flow cytometry.
10.[ Objectives ] The study was designed to investigate the HSP70 expression, cell apoptosis and the percentage of the G1 phase of cell line MRC-5 at different times, using a specific anti-sense oligodeoxynucleotides which is matched with human HSP70mRNA, and to research whether the reduced expression of HSP70 will induce the cell senescence.
