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1.Dol-ELISA was preliminarily used in the diagnosis of Clonorchiasis sinensis. Among 40 confirmed Clonorchiasis sinensis cases 82.5% showed positive reac-tions, one serum of 40 sera from healthy adults was found rositive.
以肝吸虫成虫抗原进行Dot-ELISA试验,40例粪查阳性的肝吸虫病患者血清,有33(82.5%)例呈阳性反应。收藏指正
2.The standard antigen and serum of the OPP and CAE were used to examine 550 sera of saanen milk goats, dark, white, flaxen goats.
采用关节炎脑炎 (CAE)标准抗原和血清检测萨能奶山羊、繁殖羔羊、杂交奶山羊、地方 (黑、白、麻 )山羊的血清 550份 ,检出 CAE阳性血清 30份 ,可疑 5份。收藏指正
3.Total serum protein and the concentration of IgM of the normal sera of bullhead catfish were determined by using the Coomassie blue dye binding method of Bradford and sandwich ELISA, the values being 17.57mg/ml and 1.16mg/ml, respectively. The percentage of IgM in the total serum protein was 6.60% .
以正常黄颡鱼血清IgM系列稀释液作蛋白标准，通过夹心ELISA法检测正常黄颡鱼血清中IgM的含量。 正常黄颡鱼血清中IgM和血清总蛋白含量分别为1.16mg／ml和17.57mg／ml，经过计算，正常黄颡鱼血清中IgM占血清总蛋白的比例为6.60％。收藏指正
4.Results (1) None of the sera recognized PR3 or MPO, only one serum recognized BPI.
结果（1）无一例血清识别PR3、MPO； 仅1例识别BPI；收藏指正
5.Results demonostrated (1) that the mean cord serum erythropoietin levels wrer higher than their 72-84 hour old blood samples and maternal sera both in AGA and SGA (P<0.01).
结果发现正常新生儿和小样儿的脐血红细胞生成素浓度高于生后72～84小时(P<0.01)和母血(P<0.01)。收藏指正
6.Methods: TTV DNA was detected in serum and PBMC of 66 hemodialysis patients by polymerase chain reaction (PCR). HBsAg and Anti HCV in sera was detected by EIA.
方法 :采用聚合酶链反应 (PCR)对 6 6例血液透析患者血清及PBMC进行TTV DNA检测 ,同时采用酶免疫分析 (EIA)检测血清中HBsAg和抗 HCV。收藏指正
7.Results:The serum antibody of all experimental rats could be detected in four weeks after infection. The false positive in sera against schostosoma japonicum,Trichinella spiralis,Toxocara canis/Paragonimus westermani and angiostrongylus cantonensis were 2/10,2/10,2/10,1/10和0.Conclusion:ELISA, with a high sensitivity and specificity, was fit to detect C.
结果 :大鼠在感染 4周后可全部阳性检出 ,该法对大鼠旋毛虫、日本血吸虫、犬弓蛔虫、广州管圆线虫和肺吸虫抗体阳性血清分别有 2 /10、2 /10、2 /10、1/10和 0的假阳性。收藏指正
8.Aleutian mink disease virus CIEP antigens were analysed with Western blotting. The serum from infected mink, the polyclonal antibody serum, contains antibodies against the CIEP antigens whose molecular weights are 60000, 50000 and 25000 respectively, but the monoclonal antibody sera only react with antigens with Mr 60000 and 50000.It suggested that the epitope of the specific antigen in CIEP for diagnosing Aleutian mink disease locates on viral protein with Mr 25000.
以水貂阿留申病病毒对流免疫电泳(CIEP)细胞抗原为材料,经酶印迹(Westemblotting)测定,水貂阿留申病病毒CIEI细胞抗原与多克隆阳性血清反应,分子量为60000,50000和25000,而与CIEP阴性的抗水貂阿留申病病毒的单克隆抗体(Y—2—9)反应,分子量为60000,50000.因此初步确定水貂阿留申病病毒CIEP细胞抗原决定族位于分子25000蛋白上.收藏指正
9.Methods Sera of 103 healthy controls and 108 patients with high serum ALT were collected and GDH and CHE were determined simultaneously by Bayer ADVIA1650 biochemical analyzer.
方法收集103名健康者和108例高ALT患者的血清标本,在BayerADVIA1650型全自动生化分析仪上同时测定GDH、CHE的活性。收藏指正
10.The sera of all recipients were collected at the 4th hour after transplantation, then tested the content of alanine aminotransferase (ALT), aspartate amino transferase (AST), lactate dehydrogenase (LDH), serum total bilirubin (STB) and hyaluronic acid (HA) in serum.
各组于术后4小时,检测受体血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、血清总胆红素(STB)、透明质酸(HA)的浓度;收藏指正
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