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1.In the present paper cellular and molecular biological studies on the bioeffects of extremely low frequency electromagnetic fields(ELF EMFs) were reviewed. The mentioned observation results involved effects of ELF EMFs on signal transduction, enzyme activity, gene expression, cell proliferation, differentiation, apoptosis, gene mutation and chromosome aberration.
综述关于极低频电磁场生物学效应的细胞和分子生物学研究结果 ,包括跨膜信号传递、酶活性、基因表达、细胞增殖、分化和凋亡以及基因突变和染色体畸变等方面。收藏指正
2.Objective To explore the role of whole peptidoglycan(WPG) of Bifidobacteria to activate macrophages in view of signal pathway ERK→AP-1.Method AP-1 activity of macrophages was detected by using electrophoretic mobility shift assay,after WPG stimulated peritoneal macrophages of SD mice.
目的探索信号途径ERK→AP-1在双歧杆菌的完整肽聚糖(WPG)激活巨噬细胞中的作用。 方法以WPG刺激SD大鼠腹腔巨噬细胞,采用凝胶电泳迁移分析技术检测巨噬细胞AP-1的活性。收藏指正
3.Objective: To observe the expression of signal transducer and activator of transcription 3(STAT3) and its activity in the spinal nerve and their nerve cell bodies.
目的 :研究脊神经及其胞体中信号传导子和转录激活子 3( STAT3)的表达及其活化程度。收藏指正
4.Abstract: This paper is an attempt to reviewbriefly the following fourresearch trends in plant physiology at the turn of century: from research on biological macromolecules to clarification of complex life activitiesgenomics,construction and function of gene; a key link in achieving integrated life activity signal transduction; the energy and material basis of life activity metabolism and its control; the interrelationship between plant and environment (both biological and abiological ) biological coevolution and adaptability.
文摘:简要介绍了当今植物生理学研究中值得注意的四个动向,它们是:从研究生物大分子到阐明复杂生命活动——基因组学、基因结构与功能的研究;实现生命整体性的重要环节——信号传递的研究;生命活动的能量和物质基础——代谢及其调节的研究;植物与环境(非生物和生物环境)的相互关系——生物的协同进化和适应的研究。收藏指正
5.The activity ofextracellular signal regulated-protein kinase(Eax)was determined by specific substrate phosphorylation assay.
特异性底物磷酸化法测定细胞外信号调节激酶(Eng)活性; 凝胶迁移率法检测转录活化因于(AP-1)DNA结合活性。收藏指正
6.RIM101 pathway is a conserved fungal signal transduction pathway and at least partly controls the cellular pH response through the activity of the zinc finger transcription factor Rim101p.
RIM101途经是真菌中一种保守的信号转导途径,白念珠菌也存在RIM101途经,并且该途径至少部分地控制着细胞对pH的应答。收藏指正
7.Objective To investigate the effect of janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway in mediating activity of NF-κB and level of TNF-α during ischemia-reperfusion in rat cardial muscles.
目的研究Janus激酶/信号转导和转录激活子(januskinase/signaltransducerandactivatoroftranscription,JAK/STAT)通路对缺血再灌注损伤所至大鼠心肌组织NF-κB和TNF-α表达的影响。收藏指正
8.Objective To investigate the signal transduction mechanism underlying the effects of angiotensinⅡ (AngⅡ) and aldosterone (Aldo) on nuclear factor κB(NF κB) DNA binding activity.
目的 探讨血管紧张素Ⅱ(AngⅡ)和醛固酮对肝星状细胞(HSC)核因子κB信号转导通路的影响。收藏指正
9.In addition, oxidative modification function on the activation of apoptosis-inducing factor (AIF), on the regulation of apoptosis signal-regulating kinase 1(ASK1) signaling pathway and on the activation and activity of anti-apoptosis transcription factor NF-κB. Taken together, oxidative modification appears to be a pivotal modulating fashion and novel molecular mechanism in addition to phosphorylation and ubiquition in regulation of apoptotic signaling.
氧化修饰作用也涉及凋亡诱导因子、促凋亡的凋亡信号调控激酶1(apoptosis signal-regulating kinase1,ASK1)信号转导途径及抗凋亡的转录因子NF-κB的激活和活性。 所以氧化修饰可能是调控凋亡信号转导机制中除磷酸化、泛素化外的另一个新的分子机制。收藏指正
10.The expression of IL-12 protein and STAT4 (signal transducer and act ivator of transcription, STAT) protein in liver grafts was detected by using Wes tern-blotting. DNA binding activity of STAT4 was measured by EMSA.
分别采用Western blot及EMSA检测肝移植后 0h和4d移植肝IL 12蛋白表达、STAT4蛋白表达及其DNA结合活性 ;收藏指正
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