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1.Single factor experiment and orthogonal experiment were studied on the culture conditions of stain BM01 producing flocculant, and the results suggested that sucrose was used as carbon sourse which the content of was 5‰ ,and the content of the clay mineral poweder was 2.5‰. In addition, the flocculating rate of MBF-BM01 arrived at 95.2% at pH7 of medium, when we disposed of 3‰ Kaolin clay suspension liquid.
对BM01菌株产絮凝剂的最佳培养条件进行单因子试验、正交试验,结果表明:蔗糖为碳源且含量为5‰,矿粉添加量为2.5‰,培养基初始pH值为7.0,处理3‰的高岭土悬浊液,BM01菌株产絮凝剂(MBF-BM01)的絮凝率最高为95.2%。收藏指正
2.The cortices of the rat embryos on E13d,E15d and E17d were dissected respectively. The cortices obtained were dissociated into single cells and the cell pellets were made. The pellets were stained with anti nestin and anti NeuN immunocychemical stain.
取E13d、E15d和E17d胎鼠皮质,分离获得神经细胞并制备成滴片,免疫细胞化学方法抗nestin和抗NeuN染色,观察不同胚期胎鼠皮质神经干细胞和神经元细胞比例。收藏指正
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