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1.Through DNA/DNA molecular hybridization, Nopaline as-say and NPT Ⅱ assay, it is certain that the Nos/Npt gene in T-DNA was transfer-red into the somatic embryos of Citrus reticulata Blano cv. yu dong, and expressed in the transformed embryos, and the toxic protein gene of Bacillus thuringiensis was transferred into the somatic embryos. It is the first example of introducing foreign gene into Citrus.
通过Nopalim和NPTⅡ酶性检测、DNA/DNA分子杂交鉴定,证明了Nos基因、NPTⅡ基因已整合到贡柑体胚的细胞基因组中并表达,苏云金杆菌的毒蛋白基因整合到贡柑细胞基因组中,第一次在柑桔类果树实现了外源基因转移。收藏指正
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