spermatozoa-coating antigen例句翻译_写作句库

spermatozoa-coating antigen

1.Immunizing antigen (SDL—HSA) and coating antigen (SDL—OVA) were synthesized by conjugating SDL to human serum albumin (HSA) and ovalbumin (OVA) with the same method of mixed—anhydride.

采用混合酸酐法将SDL与人血清白蛋白(HSA)连接制备了免疫抗原SDL—HSA,用同样的方法将其与卵清白蛋白(OVA)连接制备了包被抗原SDL—OVA。收藏指正

2.MPA was derived, and then coupled to bovine serum albumin (BSA) and ovalbumin (OVA) to synthesize immunogen MPA-BSA and coating antigen MPA-OVA respectively through mixed anhydride.

先将肟化后的MPA分别与牛血清白蛋白(bovine serum albumin,BSA)和卵清白蛋白(ovalbumin,OVA)通过混合酸酐法交联,制备成免疫抗原MPA-BSA和包被抗原MPA-OVA。收藏指正

3.3. An indirect ELISA using protein VP2 which was expressed by Picha Pastoris as coating antigen was established to monitor antibodies level of pigs against PPV and its operation methods were optimized.

3．通过酵母分泌表达获得的猪细小病毒VP2蛋白经透析纯化后，作为包被抗原初步建立了检测猪细小病毒抗体水平的间接ELISA方法，并对该方法进行了优化，用此优化方法检测了200份来自发病猪场的待检猪细小病毒阳性血清样品，并将其与华中农业大学的乳胶凝集试剂盒相比较，符合率达92.6％。收藏指正

4.In the inhibitory competitive ELISA ,the concentration coating antigen and antibody were determined by chess-board experiment ,the regression equation was Logit Y=-1.26LgC+4.37(R2=0.98), inter-well variation were 1.30%(n=8).

包被抗原及抗体工作浓度均由方阵实验确定。标准曲线在0.10-4.00μg／ml的范围内所测的吸光度值与Da标准品浓度的对数表现出负相关，相关系数R~2=0.98，回归方程为LogitY=-1.26IgC+4.37。收藏指正

5.The coating antigen molecules,including recombinant human augmenter of liver regeneration (ALR),double strand DNA and cardiolipin,and the coating anti-ALR antibody molecules with different status (unoxidized and oxidized antibody) were immobilized on the modified PS plates respectively and investigated by EIA. A synchronous EIA was performed to compare the results by using untreated PS plates.

用重组人增强肝脏再生因子(rhALR)、双链DNA(dsDNA)、心磷脂为包被抗原,以未氧化型与氧化型抗ALR为包被抗体,分别固定于不同修饰的PS板表面进行酶免疫分析,并与未经修饰处理的PS板作对比研究。收藏指正

6.When AFB1-OV was used as coating antigen, Fab1 as reaction antibody, and Ab2 and Fab2 as competitive antigens, the concentration of Ab2 and Fab2 was 3.98ug/ml and 1.12ug/ml at 50% competitive inhibitory ratio, respectively.

以AFB1与卵清蛋白(OV)的连接物AFB1-OV为包被抗原,Fab1为反应抗体,Ab2和Fab2为竞争抗原,达到50%的竞争抑制率时,Ab2和Fab2的浓度分别为3.98ug/ml和1.12ug/ml;收藏指正

7.Four recombinant proteins from different genotype and sub genotype of HEV ORF2 (452~617aa), including Burma strain from subgenotype I a (p166Bur), Pakistan strain from subgenotype I b (p166Pak), Mexican strain from genotype II (p166Mex), and US strain from genotype III (p166Us) , were used as coating antigen.

检测75份猪血清，p166Us抗原的阳性检出率58／75(77.3％)，高于p166Pak43／75(57.3％)、p166Mex27／75(36％)和p166Bur20／75(26.7％)，而且p166Us可以检测到的抗体滴度最高。收藏指正

8.Offered advanced technology for diagnosis of the disease of porcine parvovirus. Based on the purified recombinant proteins, an indirect ELISA method for detection of PPV antibodies was developed. The concentration of coating antigen of NS1 is 2.69μg/ml and VP2 is 2.29μg/ml, HRP labeled anti-porcine IgG (1: 40000) being incubated at 37□ for 1h.

用纯化的猪细小病毒NS1蛋白和VP2蛋白作为包被抗原包被酶标板,建立了检测猪细小病毒抗体的间接ELISA方法,抗原包被浓度NS1蛋白和VP2蛋白分别为2.69μg/mL、2.29μg/mL,二抗(1:40000)37℃作用1h,底物溶液37℃显色5min。收藏指正

9.coating application

涂料施工收藏指正

10.coating factory

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spermatozoa coating antigen